2x nonreducing sds protein gel loading solution Search Results


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New England Biolabs sequence q5 high fidelity 2x master mix
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Proteintech x bax
Expressions of autophagy- and apoptosis-related proteins are increased upon IRI. (A) Representative western blot images of <t>BAX,</t> <t>Bcl-2,</t> and CC3 expressions in renal tissue lysates of WT mice subjected to sham or I/R operations. Bars represent mean ± SEM, n = 3, * p < 0.05. (B) Representative western blot images of LC3-II and p62 expressions in renal tissue lysates of WT mice subjected to sham or I/R operations. Bars represent mean ± SEM, n = 3, * p < 0.05.
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Bio-Rad tgx stain free precast gels
Expressions of autophagy- and apoptosis-related proteins are increased upon IRI. (A) Representative western blot images of <t>BAX,</t> <t>Bcl-2,</t> and CC3 expressions in renal tissue lysates of WT mice subjected to sham or I/R operations. Bars represent mean ± SEM, n = 3, * p < 0.05. (B) Representative western blot images of LC3-II and p62 expressions in renal tissue lysates of WT mice subjected to sham or I/R operations. Bars represent mean ± SEM, n = 3, * p < 0.05.
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Addgene inc ensconsin microtubule binding domain 2x mcherry
Expressions of autophagy- and apoptosis-related proteins are increased upon IRI. (A) Representative western blot images of <t>BAX,</t> <t>Bcl-2,</t> and CC3 expressions in renal tissue lysates of WT mice subjected to sham or I/R operations. Bars represent mean ± SEM, n = 3, * p < 0.05. (B) Representative western blot images of LC3-II and p62 expressions in renal tissue lysates of WT mice subjected to sham or I/R operations. Bars represent mean ± SEM, n = 3, * p < 0.05.
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Image Search Results


Expressions of autophagy- and apoptosis-related proteins are increased upon IRI. (A) Representative western blot images of BAX, Bcl-2, and CC3 expressions in renal tissue lysates of WT mice subjected to sham or I/R operations. Bars represent mean ± SEM, n = 3, * p < 0.05. (B) Representative western blot images of LC3-II and p62 expressions in renal tissue lysates of WT mice subjected to sham or I/R operations. Bars represent mean ± SEM, n = 3, * p < 0.05.

Journal: Frontiers in Molecular Biosciences

Article Title: The Role of TRPC6 in Renal Ischemia/Reperfusion and Cellular Hypoxia/Reoxygenation Injuries

doi: 10.3389/fmolb.2021.698975

Figure Lengend Snippet: Expressions of autophagy- and apoptosis-related proteins are increased upon IRI. (A) Representative western blot images of BAX, Bcl-2, and CC3 expressions in renal tissue lysates of WT mice subjected to sham or I/R operations. Bars represent mean ± SEM, n = 3, * p < 0.05. (B) Representative western blot images of LC3-II and p62 expressions in renal tissue lysates of WT mice subjected to sham or I/R operations. Bars represent mean ± SEM, n = 3, * p < 0.05.

Article Snippet: Sources of antibodies and reagents are as follows: fetal bovine serum (FBS) (Invitrogen), DMEM/F12 (Invitrogen), anti-cleaved caspase 3 (Cat#: 9661, Cell Signaling Technology), anti-Bcl-2 (Cat#: 12789-1-AP, Proteintech), anti-Bcl-2–associated X (BAX) (Cat#: 50599-2, Proteintech), p-AKT (ser473) (Cat#: 4060P, Cell Signaling Technology), AKT (Cat#: 9272, Cell Signaling Technology), p-ERK1/2 (Cat#: 4370, Cell Signaling Technology), ERK1/2 (Cat#: 4695, Cell Signaling Technology), TRPC6 (Cat#: ACC-017, Alomone), polycystin 2 (Cat#: 19126-1-AP, Proteintech), AURKA (Cat#: 66757-1-lg, Proteintech), HIF-1α (Cat#: 79233, Cell Signaling Technology), p62 (Cat#: 88588, Cell Signaling Technology), β-actin (Cat#: TA-09, ZhongShan Biotechnology), HRP-conjugated anti-rabbit IgG (Cat#: 110777, KeRui Biotechnology), HRP-conjugated anti-mouse IgG (Cat#: 117228, KeRui Biotechnology), U0126 (Cat#: HY-12-31, MedChemExpress), MK2206 (Cat#: HY-10358, MedChemExpress), thapsigargin (Tg) (Cat#: T7459, Invitrogen), JC-1 dye (Bio-Swamp), Creatinine (Cr) Assay Kit (sarcosine oxidase) (Cat#: C011-2, Nanjing Jiancheng), Urea Assay Kit (urease) (Cat#: C013-2, Nanjing Jiancheng), and OCT compound (4583, Sakura).

Techniques: Western Blot

Autophagy is augmented and apoptosis is mitigated in TRPC6 −/− mice upon I/R treatment. (A) Representative western blot images of BAX, Bcl-2, and CC3 expressions in renal tissue lysates extracted from WT and TRPC6 −/− mice subjected to I/R operations. Data are expressed as mean ± SEM, n = 3, * p < 0.05. (B) Representative western blot images of LC3-II and p62 expressions in renal tissue lysates extracted from WT and TRPC6 −/− mice subjected to I/R operations. Data are expressed as mean ± SEM, n = 3, * p < 0.05. (C) Representative confocal microscopy images of CC3 expression in kidney sections from WT and TRPC6 −/− mice subjected to sham or I/R operations (photographed at a magnification of ×200, scale bar: 50 μm). Bars represent mean ± SEM, n = 6, * p < 0.05.

Journal: Frontiers in Molecular Biosciences

Article Title: The Role of TRPC6 in Renal Ischemia/Reperfusion and Cellular Hypoxia/Reoxygenation Injuries

doi: 10.3389/fmolb.2021.698975

Figure Lengend Snippet: Autophagy is augmented and apoptosis is mitigated in TRPC6 −/− mice upon I/R treatment. (A) Representative western blot images of BAX, Bcl-2, and CC3 expressions in renal tissue lysates extracted from WT and TRPC6 −/− mice subjected to I/R operations. Data are expressed as mean ± SEM, n = 3, * p < 0.05. (B) Representative western blot images of LC3-II and p62 expressions in renal tissue lysates extracted from WT and TRPC6 −/− mice subjected to I/R operations. Data are expressed as mean ± SEM, n = 3, * p < 0.05. (C) Representative confocal microscopy images of CC3 expression in kidney sections from WT and TRPC6 −/− mice subjected to sham or I/R operations (photographed at a magnification of ×200, scale bar: 50 μm). Bars represent mean ± SEM, n = 6, * p < 0.05.

Article Snippet: Sources of antibodies and reagents are as follows: fetal bovine serum (FBS) (Invitrogen), DMEM/F12 (Invitrogen), anti-cleaved caspase 3 (Cat#: 9661, Cell Signaling Technology), anti-Bcl-2 (Cat#: 12789-1-AP, Proteintech), anti-Bcl-2–associated X (BAX) (Cat#: 50599-2, Proteintech), p-AKT (ser473) (Cat#: 4060P, Cell Signaling Technology), AKT (Cat#: 9272, Cell Signaling Technology), p-ERK1/2 (Cat#: 4370, Cell Signaling Technology), ERK1/2 (Cat#: 4695, Cell Signaling Technology), TRPC6 (Cat#: ACC-017, Alomone), polycystin 2 (Cat#: 19126-1-AP, Proteintech), AURKA (Cat#: 66757-1-lg, Proteintech), HIF-1α (Cat#: 79233, Cell Signaling Technology), p62 (Cat#: 88588, Cell Signaling Technology), β-actin (Cat#: TA-09, ZhongShan Biotechnology), HRP-conjugated anti-rabbit IgG (Cat#: 110777, KeRui Biotechnology), HRP-conjugated anti-mouse IgG (Cat#: 117228, KeRui Biotechnology), U0126 (Cat#: HY-12-31, MedChemExpress), MK2206 (Cat#: HY-10358, MedChemExpress), thapsigargin (Tg) (Cat#: T7459, Invitrogen), JC-1 dye (Bio-Swamp), Creatinine (Cr) Assay Kit (sarcosine oxidase) (Cat#: C011-2, Nanjing Jiancheng), Urea Assay Kit (urease) (Cat#: C013-2, Nanjing Jiancheng), and OCT compound (4583, Sakura).

Techniques: Western Blot, Confocal Microscopy, Expressing

TRPC6 knockout reduces TEC apoptosis by down-regulating PI3K/AKT/mTOR and ERK1/2 pathways. (A) Representative western blot images show the level of phosphorylated and total AKT and ERK1/2 expressions of tissue lysates extracted from WT and TRPC6 −/− mice. Bars represent mean ± SEM, n = 3, * p < 0.05. (B, C) Representative western blot images show the level of phosphorylated and total AKT and ERK1/2, BAX, Bcl-2, and CC3 expressions after primary TECs subjected to H/R treatment and addition with inhibitors of AKT (MK2206) and ERK (U0126). (D) Bar graphs show the relative quantification of p-AKT/AKT, p-ERK/ERK, CC3/β-actin, and BAX/Bcl-2. Data are expressed as mean ± SEM, n = 3, * p < 0.05.

Journal: Frontiers in Molecular Biosciences

Article Title: The Role of TRPC6 in Renal Ischemia/Reperfusion and Cellular Hypoxia/Reoxygenation Injuries

doi: 10.3389/fmolb.2021.698975

Figure Lengend Snippet: TRPC6 knockout reduces TEC apoptosis by down-regulating PI3K/AKT/mTOR and ERK1/2 pathways. (A) Representative western blot images show the level of phosphorylated and total AKT and ERK1/2 expressions of tissue lysates extracted from WT and TRPC6 −/− mice. Bars represent mean ± SEM, n = 3, * p < 0.05. (B, C) Representative western blot images show the level of phosphorylated and total AKT and ERK1/2, BAX, Bcl-2, and CC3 expressions after primary TECs subjected to H/R treatment and addition with inhibitors of AKT (MK2206) and ERK (U0126). (D) Bar graphs show the relative quantification of p-AKT/AKT, p-ERK/ERK, CC3/β-actin, and BAX/Bcl-2. Data are expressed as mean ± SEM, n = 3, * p < 0.05.

Article Snippet: Sources of antibodies and reagents are as follows: fetal bovine serum (FBS) (Invitrogen), DMEM/F12 (Invitrogen), anti-cleaved caspase 3 (Cat#: 9661, Cell Signaling Technology), anti-Bcl-2 (Cat#: 12789-1-AP, Proteintech), anti-Bcl-2–associated X (BAX) (Cat#: 50599-2, Proteintech), p-AKT (ser473) (Cat#: 4060P, Cell Signaling Technology), AKT (Cat#: 9272, Cell Signaling Technology), p-ERK1/2 (Cat#: 4370, Cell Signaling Technology), ERK1/2 (Cat#: 4695, Cell Signaling Technology), TRPC6 (Cat#: ACC-017, Alomone), polycystin 2 (Cat#: 19126-1-AP, Proteintech), AURKA (Cat#: 66757-1-lg, Proteintech), HIF-1α (Cat#: 79233, Cell Signaling Technology), p62 (Cat#: 88588, Cell Signaling Technology), β-actin (Cat#: TA-09, ZhongShan Biotechnology), HRP-conjugated anti-rabbit IgG (Cat#: 110777, KeRui Biotechnology), HRP-conjugated anti-mouse IgG (Cat#: 117228, KeRui Biotechnology), U0126 (Cat#: HY-12-31, MedChemExpress), MK2206 (Cat#: HY-10358, MedChemExpress), thapsigargin (Tg) (Cat#: T7459, Invitrogen), JC-1 dye (Bio-Swamp), Creatinine (Cr) Assay Kit (sarcosine oxidase) (Cat#: C011-2, Nanjing Jiancheng), Urea Assay Kit (urease) (Cat#: C013-2, Nanjing Jiancheng), and OCT compound (4583, Sakura).

Techniques: Knock-Out, Western Blot