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Image Search Results
Journal: Frontiers in Molecular Biosciences
Article Title: The Role of TRPC6 in Renal Ischemia/Reperfusion and Cellular Hypoxia/Reoxygenation Injuries
doi: 10.3389/fmolb.2021.698975
Figure Lengend Snippet: Expressions of autophagy- and apoptosis-related proteins are increased upon IRI. (A) Representative western blot images of BAX, Bcl-2, and CC3 expressions in renal tissue lysates of WT mice subjected to sham or I/R operations. Bars represent mean ± SEM, n = 3, * p < 0.05. (B) Representative western blot images of LC3-II and p62 expressions in renal tissue lysates of WT mice subjected to sham or I/R operations. Bars represent mean ± SEM, n = 3, * p < 0.05.
Article Snippet: Sources of antibodies and reagents are as follows: fetal bovine serum (FBS) (Invitrogen), DMEM/F12 (Invitrogen), anti-cleaved caspase 3 (Cat#: 9661, Cell Signaling Technology), anti-Bcl-2 (Cat#: 12789-1-AP, Proteintech), anti-Bcl-2–associated
Techniques: Western Blot
Journal: Frontiers in Molecular Biosciences
Article Title: The Role of TRPC6 in Renal Ischemia/Reperfusion and Cellular Hypoxia/Reoxygenation Injuries
doi: 10.3389/fmolb.2021.698975
Figure Lengend Snippet: Autophagy is augmented and apoptosis is mitigated in TRPC6 −/− mice upon I/R treatment. (A) Representative western blot images of BAX, Bcl-2, and CC3 expressions in renal tissue lysates extracted from WT and TRPC6 −/− mice subjected to I/R operations. Data are expressed as mean ± SEM, n = 3, * p < 0.05. (B) Representative western blot images of LC3-II and p62 expressions in renal tissue lysates extracted from WT and TRPC6 −/− mice subjected to I/R operations. Data are expressed as mean ± SEM, n = 3, * p < 0.05. (C) Representative confocal microscopy images of CC3 expression in kidney sections from WT and TRPC6 −/− mice subjected to sham or I/R operations (photographed at a magnification of ×200, scale bar: 50 μm). Bars represent mean ± SEM, n = 6, * p < 0.05.
Article Snippet: Sources of antibodies and reagents are as follows: fetal bovine serum (FBS) (Invitrogen), DMEM/F12 (Invitrogen), anti-cleaved caspase 3 (Cat#: 9661, Cell Signaling Technology), anti-Bcl-2 (Cat#: 12789-1-AP, Proteintech), anti-Bcl-2–associated
Techniques: Western Blot, Confocal Microscopy, Expressing
Journal: Frontiers in Molecular Biosciences
Article Title: The Role of TRPC6 in Renal Ischemia/Reperfusion and Cellular Hypoxia/Reoxygenation Injuries
doi: 10.3389/fmolb.2021.698975
Figure Lengend Snippet: TRPC6 knockout reduces TEC apoptosis by down-regulating PI3K/AKT/mTOR and ERK1/2 pathways. (A) Representative western blot images show the level of phosphorylated and total AKT and ERK1/2 expressions of tissue lysates extracted from WT and TRPC6 −/− mice. Bars represent mean ± SEM, n = 3, * p < 0.05. (B, C) Representative western blot images show the level of phosphorylated and total AKT and ERK1/2, BAX, Bcl-2, and CC3 expressions after primary TECs subjected to H/R treatment and addition with inhibitors of AKT (MK2206) and ERK (U0126). (D) Bar graphs show the relative quantification of p-AKT/AKT, p-ERK/ERK, CC3/β-actin, and BAX/Bcl-2. Data are expressed as mean ± SEM, n = 3, * p < 0.05.
Article Snippet: Sources of antibodies and reagents are as follows: fetal bovine serum (FBS) (Invitrogen), DMEM/F12 (Invitrogen), anti-cleaved caspase 3 (Cat#: 9661, Cell Signaling Technology), anti-Bcl-2 (Cat#: 12789-1-AP, Proteintech), anti-Bcl-2–associated
Techniques: Knock-Out, Western Blot